Table 2. Crystallization conditions.
MPD, 2-methyl-2,4-pentanediol; MgAc, magnesium acetate tetrahydrate.
| 34-mer RNA | 34-mer DNA | ||||
|---|---|---|---|---|---|
| Crystal | Form 1 | Form 2 | Form 1 | Form 2 | 45-mer DNA |
| Droplet | |||||
| Sample (mM) | 0.25 | 0.25 | 0.5 | 0.5 | 0.5 |
| Na cacodylate (mM) | 25 (pH 6.5) | 25 (pH 6.5) | 20 (pH 6.0) | 40 (pH 6.0) | 25 (pH 7.0) |
| Spermine.4HCl (mM) | 6 | 6 | 10 | 13 | 5 |
| NaCl (mM) | — | — | 6 | 50 | 50 |
| KCl (mM) | — | — | 60 | 50 | — |
| Mg2+ (mM) | 5 (MgSO4) | 5 (MgAc) | — | — | 50 (MgCl2) |
| (NH4)2SO4 (M) | 1 | — | — | — | — |
| Li2SO4·H2O (M) | — | 0.7 | — | — | — |
| MPD (%) | — | — | 5 | 2 | 5 |
| Reservoir | |||||
| MPD (%) | — | — | 20 (1 ml) | 8 (1 ml) | 30 (1 ml) |
| (NH4)2SO4 (M) | 2 (0.7 ml) | — | — | — | — |
| Li2SO4·H2O (M) | — | 1.3 (0.7 ml) | — | — | — |
| Temperature (K) | 310 | 310 | 277 | 277 | 277 |