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Elution profiles from a Superdex 200 gel-filtration column (GE Healthcare) of (a) YwfE and (b) molecular-mass standard proteins in 50 mM Tris–HCl pH 8.0 and 0.15 M NaCl. Inset in (a): SDS–PAGE (10% acrylamide gel) analysis of purified YwfE protein (3 µg) with Coomassie Blue staining. The molecular masses of the standard proteins are indicated in kDa.
