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. 2012 Jan 20;9:15. doi: 10.1186/1742-2094-9-15

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Copyright ©2012 Ellrichmann et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FACS phenotying of lysMCreIκBα^fl/flmice. (A) Analysis of spleen cells from naïve lysMCreIκBα^fl/flmice (n = 7) and wild-type mice (n = 8). Isotype controls are coloured in red, CD11b+ cell fractions are marked with a yellow box. (B) FACS analyses show a significantly increased number of spleen-derived CD11b positive monocytes and CD11b/Ly6c double positive cells in lysMCreIκBα^fl/flmice. Data are shown in per cent of total cell count. (C) lysMCreIκBα^fl/flmice display a non significant increase of the co-stimulatory molecules CD80 and CD86. While there is no effect on MHC-II (C), there is a significant increase in Ly6c expression on CD11b+ cells from lysMCreIκBα^fl/flmice (D).