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. 2012 Feb 7;10(2):e1001256. doi: 10.1371/journal.pbio.1001256

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Trichas et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) At left, diagram illustrating the mouse egg-cylinder, with the ExE-VE in blue (situated proximally) and Epi-VE in light green (situated distally). AVE cells are marked in dark green. At right, high magnification views of the VE of an egg-cylinder stage mouse embryo stained with ZO-1, showing differences in cell shape in the ExE-VE and Epi-VE. Scale bars = 25 µm. (B) The mean polygon number in the ExE-VE and Epi-VE at different wild-type stages (“pre-AVE”: before AVE induction, n = 337 Epi-VE and 231 ExE-VE cells from four embryos; “distal”: AVE at distal tip before migration, n = 497 Epi-VE and 396 ExE-VE cells from four embryos; “migrating”: AVE migrating, n = 300 Epi-VE and 236 ExE-VE cells from three embryos; and “anterior”: AVE finished proximal migration and moving laterally, n = 480 Epi-VE and 409 ExE-VE cells from three embryos) and in the AVE arrest mutants Nodal^Δ600/lacZ (n = 724 Epi-VE and 565 ExE-VE cells from five embryos) and Cripto ^−/− (n = 605 Epi-VE and 598 ExE-VE cells from five embryos). At “migrating” and “anterior” stages, the mean polygon number in the Epi-VE is significantly lower than that in the ExE-VE. AVE arrest mutants isolated at a stage comparable to “anterior” embryos do not show this significant reduction in polygon number in the Epi-VE. (B′) The same polygon number data grouped according to the VE region. In the Epi-VE, mean polygon number in “migrating” and “anterior” embryos is significantly lower than that in “distal” embryos. The Epi-VE of AVE arrest mutants has a mean polygon number that is significantly different to that of stage matched “anterior” embryos but more similar to that of “distal” embryos. (C) Mean polygon numbers of Epi-VE (n = 31) and ExE-VE (n = 28) cells in five cultured embryos, measured at the start of and during AVE migration. The mean polygon number of the Epi-VE cells reduced significantly during migration, while that of the ExE-VE cells did not. p values shown on the graphs were determined using Student's t test.