Table 1.

Effect of 6H5 mAb on growth of breast cancer cells^*

Cell line	Mean No. of cells
		Dose 0 vs 1				Dose 0 vs 2				Dose 0 vs 3
	Dose 0	Dose 1	Difference	95% CI	P†	Dose 2	Difference	95% CI	P†	Dose 3	Difference	95% CI	P†	N
MCF-10AT	36 667	48 333	−11 667	−50 652 to 27 318	.45	53 333	−16 667	−73 142 to 39 809	.46	43 333	−6667	−48 818 to 35 484	.68	3
MCF-7	261 667	113 333	148 333	89 628 to 207 039	.002	96 667	165 000	109 096 to 220 904	.001	91 667	170 000	113 524 to 226 476	.001	3
MDA-MB-231	146 667	80 000	66 667	44 966 to 88 368	.001	66 667	80 000	62 689 to 97 311	<.001	56 667	90 000	70 924 to 109 076	<.001	3
SKBR3	143 833	83 333	60 500	−43 213 to 164 213	.18	60 000	83 833	5659 to 162 008	.041	47 500	96 333	14 841 to 177 826	.031	3
IDCm73	81 667	63 333	18 333	5247 to 31 420	.018	48 333	33 333	22988 to 43679	<.001	41 667	40 000	26 914 to 53 086	.001	3

^*The nonmalignant breast cell line MCF-10AT; breast cancer cell lines MCF-7, MDA-MB-231, and SKBR3; and a cell line cultured from the primary tumor of a breast cancer patient (IDCm73T) were left untreated (dose 0), were dosed with 6H5 mAb (10 μg/mL/dose), and continuously exposed for to mAb for 24 hours (dose 1), were treated at 24 and 48 hours and continuously exposed to mAb (dose 2) or were treated at 24, 48, and 72 hours and continuously exposed to mAb (dose 3). Cells were stained with trypan blue and living cells were counted from four fields under low power microscopy. Mean values from at least three independent experiments are presented. CI = confidence interval; mAb = monoclonal antibodies; N = number of independent experiments.

^†P values were calculated using a two-sided Student's test.