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. 2012 Feb 7;7(2):e30630. doi: 10.1371/journal.pone.0030630

Figure 2. Gbrowse snapshot showing examples of mis-predictions in the current T. thermophila genome annotation that were corrected by RNA-seq.

Figure 2

A, mis-predicted gene TTHERM_00257230 with incorrect intron boundary; note that original gene annotations did not include UTRs. B, a region previously annotated as containing five predicted genes has only one gene; C, a region previously annotated as one predicted gene (TTHERM_00130000) contains two genes, as further indicated by the coverage discontinuity between the two assembled transcripts strongly argues for two genes. D, a gene prediction with multiple errors, including a mis-predicted splice site (red box), spurious exons and an incorrect gene fusion. There is good functional annotation support for these two transcripts: their top BLASTP matches are to “tubulin tyrosine ligase (E-value: 1e-113)” and “serine carboxypeptidase S28 family protein (E-value: 3e-110)” respectively.