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. 2012 Feb 7;7(2):e31454. doi: 10.1371/journal.pone.0031454

Figure 2. Effect of heparinase I treatment on MAV-1 and Ad5 attachment to A549 cells.

Figure 2

A. FACS analysis to assess the effect of heparinase I treatment (0 or 10 U/ml during 90 min at 37°C) on heparan sulfate expression in A549 cells. Fluorescence intensity is plotted against the number of events (counts). The green line indicates untreated A549; the red line represents heparinase I-treated A549. Background in the absence of primary antibody F58-10E4 is represented by the solid blue histogram. B. Binding experiments in A549 cells (pretreated with heparinase I as described in A) were performed as in Fig. 1. Binding capacities are presented as the percentage attached virus particles relative to the total amount of added virus particles. Means (±SEM) of three independent experiments are shown (each condition performed in duplicate). *, P<0.05 versus binding in the condition 0 U/ml heparinase I.