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. 2012 Feb 7;7(2):e31174. doi: 10.1371/journal.pone.0031174

Figure 5. NKT cells require PKC-θ in order to produce cytokines.

Figure 5

A) WT (black bars) and PKC-θ−/− (open bars) mice were challenged with OCH (1 µg) for 1 h, indicated cytokine levels in serum were detected (*, P<0.05; **, P<0.01). B. Mice were challenged with OCH (1 µg) for 1 h, IFNγ(left panel) and IL-4 (left panel) in WT (solid line) and PKC-θ−/− (dotted line) NKT cells were detected by intracellular staining of intrahepatic lymphocytes. Shaded area is isotype (ISO) antibody control. C) Intracelluar IFNγ and IL-4 levels in NKT cells described in B are indicated by GeoMean obtained from at least three independent experiments. D) Sorted NKT cells (CD3LowCD1d-PBS57+) were stimulated with 100 ng/ml OCH in vitro for overnight, and IFNγ and IL-4 levels in supernatant were measured (*, P<0.05; **, P<0.01).