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. 2012 Feb 7;7(2):e31286. doi: 10.1371/journal.pone.0031286

Figure 1. CAPE suppresses proliferation and colony formation of PC-3 cells.

Figure 1

Proliferation of PC-3 cell treated with increasing concentration of CAPE was determined by Trypan blue staining after 72 h treatment (A) or measuring total DNA content per well using Hoechst 33258 fluorescence by 96-well proliferation assay after 24, 48, and 72 h treatment (B). Relative cell numbers were normalized to the average cell number of the control (no CAPE treatment) of each cell line in each individual experiment. Columns represent mean for 18 replicates; bars represent standard deviation. Asterisk (*) represents cell number is statistically significantly different (p<0.05) compared to the control. Columns represent mean for 5 biological replicates; bars represent standard deviation. (C) Anticancer effect of CAPE was determined by colony formation assay of PC-3 cells treated with 0, 10, 20 µM for 14 days. Image is a representative result of three biological replicates.