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. 2012 Feb 7;7(2):e31286. doi: 10.1371/journal.pone.0031286

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Lin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Protein levels of wild type PC-3, PC-3 cells transfect with scramble control (20 nM), and PC-3 cells transfected with p21^Cip1 siRNA (20 nM) were determined by Western blotting assay. Proliferation of these PC-3 cells treated with 20 µM CAPE for 24 h was determined by 96-well plate proliferation assay as described in Material and Methods.