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. 2012 Feb 7;7(2):e30280. doi: 10.1371/journal.pone.0030280

Figure 5. Effect of IL-18 on Th2 differentiation and GATA3 expression in naïve CD4+ T-cells.

Figure 5

(A,B,C). Effect of NEMO peptide on Th2 differentiation. Negatively selected naïve CD3+ CD4+ T-cells were cultured with plate bound anti-CD3 and anti-CD28 with PBS (PBS), IL-18 100 ng/ml (IL-18), IL-18 100 ng/ml and 10 µM NEMO Binding Domain Binding Peptide (IL-18+NEMO) for the initial culture period. The cells were washed and restimulated, and secretion of IL-4 (A), IL-5 (B) and IFN-γ(C) were quantified. The data was expressed as fold increase compared to cells cultured with PBS. (D,E,F). GATA3 expression in T-cells. Negatively selected naïve CD3+ CD4+ T-cells were cultured with plate bound anti-CD3 and anti-CD28 in the presence of PBS (D), IL-4 (E) or IL-18 (F) for 7 days. GATA3 expression was measured by flow cytometric analysis.