IFN-α and chemokine responses to RepliVAX WN immunization. Groups of 5 B6 and IFNAR−/− mice were immunized i.p. with 3.0 × 107 IU of RepliVAX WN and serum was collected at the indicted times. The IFN-α concentration (A) was determined by ELISA. (* p < 0.03, ** p < 0.0003 compared to same time points for B6-IFNAR−/− mice) The limit of detection for IFNα was 12.5 pg/ml. The concentration of CCL2 (B) (* p < 0.007, ** p < 0.0005 compared to same time points for IFNAR−/− mice), CCL5 (C) (* p < 0.05 compared to same time point for IFNAR−/− mice), and CXCL1 (D) (* p < 0.006 compared to same time point for IFNAR−/− mice) were measured by a luminescence-based multiplex bead assay. Results are representative of 2 separate experiments. The limits of detection for CCL2, CCL5, and CXCL1 were 14.0, 5.0, and 3.0 pg/ml, respectively.