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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: Arch Biochem Biophys. 2012 Jan 3;518(2):103–110. doi: 10.1016/j.abb.2011.12.023

Figure 3. The effect of VHL and VHL-P81S on HP1, H3K9me3, ubiquitinated H2A, ubiquitinated H2B, and ubiquitinated cellular protein levels.

Figure 3

VHL-null 786-O renal carcinoma cells were infected with lentiviral vectors expressing FLAG-tagged VHL, VHL-P81S, or an empty vector. After puromycin selection, the levels of HP1, lysine-9 tri-methylated histone H3, ubiquitinated histone H2A, ubiquitinated histone H2B, and ubiquitinated cellular proteins were examined by immunoblotting. The expression levels of FLAG-VHL and FLAG-VHL-P81S were also examined by anti-FLAG immunoblotting. Tubulin serves as a loading control.