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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: Arch Biochem Biophys. 2012 Jan 3;518(2):103–110. doi: 10.1016/j.abb.2011.12.023

Figure 4. The interaction of VHL and VHL-P81S with E3 ligase components.

Figure 4

293T cells were transfected with FLAG-vector, FLAG-VHL, or FLAG-VHL-P81S. Forty-eight hours after transfection, the interaction of FLAG-VHL or FLAG-VHL-P81S with cullin 2, elongin B, and elongin C was examined by anti-FLAG immunoprecipitation followed by anti-cullin 2, elongin B, and elongin C immunoblotting.