Table 1. Proteins identified in the GST-VHL pull-down sample.
The table lists the proteins identified in the GST-VHL pull-down sample with a Protein Prophet probability score of 0.9 or higher. Entries from GST, keratin and trypsin were omitted.
| Number of Peptides |
||||||||
|---|---|---|---|---|---|---|---|---|
| Entry | Protein Name/Peptide Sequences | Peptide Charge State |
Sequence Coverage (%) |
Unique | Total | ProteinProphet Score |
Accession Number (IPI-Hum | |
| 1 | HP1 gamma | 37.2 | 5 | 8 | 1 | IPI00297579 | ||
| GFTDADNTWEPEENLDCPELIEAFLNSQK | 3 | |||||||
| LTWHSCPEDEAQ | 2 | |||||||
| WKDSDEADLVLAK | 3 | |||||||
| VEEAEPEEFVVEK | 2 | |||||||
| KVEEAEPEEFVVEK | 3 | |||||||
| 2 | HP1 beta | 26.4 | 1 | 1 | 0.98 | IPI00010320 | ||
| GFSDEDNTWEPEENLDCPDLIAEFLQSQK | 3 | |||||||
| 3 | VHL | 53.8 | 10 | 46 | 1 | IPI00027969 | ||
| VVLPVWLNFDGEPQPYPTLPPGTGR | 2 | |||||||
| VVLPVWLNFDGEPQPYPTLPPGTGR | 3 | |||||||
| SLYEDLEDHPNVQK | 2 | |||||||
| SLYEDLEDHPNVQK | 3 | |||||||
| SLYEDLEDHPNVQKDLER | 2 | |||||||
| SVNSREPSQVIFCNR | 3 | |||||||
| RLDIVR | 1 | |||||||
| SLVKPENYR | 2 | |||||||
| GHLWLFR | 2 | |||||||
| EPSQVIFCNR | 2 | |||||||
| IHSYR | 1 | |||||||
| RIHSYR | 2 | |||||||
| 4 | 60S ribosomal protein L5 | 12.5 | 3 | 4 | 1 | IPI00000494 | ||
| YLMEEDEDAYKK | 2 | |||||||
| HIMGQNVADYMR | 2 | |||||||
| HIMGQNVADYMR | 3 | |||||||
| NSVTPDMMEEMYK | 2 | |||||||
| 5 | Peroxiredoxin-1 | 58.8 | 3 | 5 | 1 | IPI00000874 | ||
| HGEVCPAGWKPGSDTIKPDVQK | 3 | |||||||
| LVQAFQFTDK | 2 | |||||||
| GLFIIDDK | 2 | |||||||
| IGHPAPNFK | 3 | |||||||
| ADEGISFR | 2 | |||||||
| 6 | HSPA5 | 13.3 | 8 | 8 | 1 | IPI00003362 | ||
| SQIFSTASDNQPTVTIK | 2 | |||||||
| VTHAVVTVPAYFNDAQR | 3 | |||||||
| SQIFSTASDNQPTVTIK | 3 | |||||||
| DAGTIAGLNVMR | 2 | |||||||
| ITPSYVAFTPEGER | 2 | |||||||
| ITPSYVAFTPEGER | 3 | |||||||
| NELESYAYSLK | 2 | |||||||
| NQLTSNPENTVFDAKR | 3 | |||||||
| 7 | HSPA8 | 32.9 | 5 | 6 | 1 | IPI00003865 | ||
| HWPFMVVNDAGRPK | 3 | |||||||
| NQVAMNPTNTVFDAK | 2 | |||||||
| TTPSYVAFTDTER | 2 | |||||||
| DAGTIAGLNVLR | 2 | |||||||
| SFYPEEVSSMVLTK | 2 | |||||||
| NQVAMNPTNTVFDAKR | 3 | |||||||
| 8 | LanC-like protein 1 | 24.6 | 7 | 12 | 1 | IPI00005724 | ||
| IPQSHIQQICETILTSGENLAR | 3 | |||||||
| FAEWCLEYGEHGCR | 3 | |||||||
| SLAEGYFDAAGR | 2 | |||||||
| LHSLVKPSVDYVCQLK | 3 | |||||||
| IDPHAPNEMLYGR | 3 | |||||||
| IRELLQQMER | 2 | |||||||
| IRELLQQMER | 3 | |||||||
| AFPNPYADYNK | 2 | |||||||
| 9 | Histone H2B type 1-L | 34.1 | 3 | 5 | 1 | IPI00018534 | ||
| AMGIMNSFVNDIFER | 2 | |||||||
| KESYSVYVYK | 2 | |||||||
| QVHPDTGISSK | 3 | |||||||
| EIQTAVR | 1 | |||||||
| 10 | F-actin-capping protein subunit beta | 26.2 | 3 | 3 | 1 | IPI00026185 | ||
| SPWSNKYDPPLEDGAMPSAR | 3 | |||||||
| QMEKDETVSDCSPHIANIGR | 3 | |||||||
| SGSGTMNLGGSLTR | 2 | |||||||
| 11 | Histone H1.2 | 17.8 | 4 | 4 | 1 | IPI00217465 | ||
| ALAAAGYDVEKNNSR | 3 | |||||||
| KASGPPVSELITK | 3 | |||||||
| ALAAAGYDVEK | 2 | |||||||
| SGVSLAALKK | 2 | |||||||
| 12 | Carbonyl reductase [NADPH] 1 | 66.8 | 19 | 54 | 1 | IPI00295386 | ||
| GQAAVQQLQAEGLSPR | 2 | |||||||
| GQAAVQQLQAEGLSPR | 3 | |||||||
| SPEEGAETPVYLALLPPDAEGPHGQFVSEK | 3 | |||||||
| VADPTPFHIQAEVTMK | 2 | |||||||
| FRSETITEEELVGLMNK | 3 | |||||||
| FHQLDIDDLQSIR | 3 | |||||||
| VVNVSSIMSVR | 2 | |||||||
| DVCTELLPLIKPQGR | 2 | |||||||
| ILLNACCPGWVR | 2 | |||||||
| FHQLDIDDLQSIR | 3 | |||||||
| LFSGDVVLTAR | 2 | |||||||
| SETITEEELVGLMNK | 2 | |||||||
| EGWPSSAYGVTK | 2 | |||||||
| DVCTELLPLIKPQGR | 3 | |||||||
| GIGLAIVR | 2 | |||||||
| SETITEEELVGLMNK | 3 | |||||||
| TNFFGTR | 2 | |||||||
| EYGGLDVLVNNAGIAFK | 2 | |||||||
| VVNVSSIMSVR | 1 | |||||||
| 13 | Histone H4 | 53.4 | 7 | 13 | 1 | IPI00453473 | ||
| TLYGFGG | 1 | |||||||
| VLRDNIQGITKPAIR | 3 | |||||||
| ISGLIYEETR | 2 | |||||||
| DAVTYTEHAK | 2 | |||||||
| TVTAMDVVYALKR | 2 | |||||||
| DNIQGITKPAIR | 2 | |||||||
| DAVTYTEHAK | 1 | |||||||
| TVTAMDVVYALK | 2 | |||||||
| 14 | ACTB Actin, cytoplasmic 1 | 25.6 | 7 | 9 | 0.99 | IPI00021439 | ||
| TTGIVMDSGDGVTHTVPIYEGYALPHAILR | 3 | |||||||
| HQGVMVGMGQK | 2 | |||||||
| AGFAGDDAPR | 2 | |||||||
| EITALAPSTMK | 2 | |||||||
| SYELPDGQVITIGNER | 2 | |||||||
| VAPEEHPVLLTEAPLNPK | 3 | |||||||
| EITALAPSTMK | 2 | |||||||
| 15 | SLC3A2 solute carrier family 3, member 2 isoform a | 7.6 | 2 | 2 | 1 | IPI00027493 | ||
| ADLLLSTQPGREEGSPLELER | 3 | |||||||
| IGDLQAFQGHGAGNLAGLK | 3 | |||||||
| 16 | CD44 antigen | 8.3 | 3 | 3 | 1 | IPI00297160 | ||
| YGFIEGHVVIPR | 3 | |||||||
| TEAADLCK | 2 | |||||||
| ALSIGFETCR | 2 | |||||||
| 17 | SLC2A1 Solute carrier family 2 | 6.2 | 2 | 2 | 1 | IPI00220194 | ||
| GTADVTHDLQEMKEESR | 3 | |||||||
| TFDEIASGFR | 2 | |||||||
| 18 | 40S ribosomal protein S15a | 17.7 | 1 | 2 | 0.99 | IPI00221091 | ||
| HGYIGEFEIIDDHR | 3 | |||||||
| WQNNLLPSR | 2 | |||||||
| 19 | Nucleolin | 3 | 2 | 3 | 0.99 | IPI00444262 | ||
| NDLAVVDVR | 2 | |||||||
| TGISDVFAK | 2 | |||||||
| 20 | Vesicle-associated membrane protein-associated protein B/C | 5.1 | 1 | 1 | 0.99 | IPI00006211 | ||
| NVCFK | 1 | |||||||
| 21 | RBPMS Isoform A of RNA-binding protein with multiple splicing | 12.6 | 1 | 1 | 0.98 | IPI00004045 | ||
| AEKENTPSEANLQEEEVR | 3 | |||||||
| 22 | 60S ribosomal protein L21 | 11.2 | 1 | 1 | 0.97 | IPI00247583 | ||
| VYNVTQHAVGIVVNK | 3 | |||||||
| 23 | Elongation factor 1-beta | 13.8 | 2 | 2 | 0.95 | IPI00178440 | ||
| SIQADGLVWGSSK | 2 | |||||||
| SSILLDVKPWDDETDMAK | 3 | |||||||
| 24 | SON protein | 1.9 | 2 | 2 | 0.92 | IPI00000192 | ||
| SMMSSYSAADR | 2 | |||||||
| SMMSPMAER | 2 | |||||||