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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Mol Ecol Resour. 2011 Dec 4;12(2):238–246. doi: 10.1111/j.1755-0998.2011.03093.x

Figure 1.

Figure 1

Diagram of nested PCR and bloodmeal identification assay. Primers (AvTrpF1, MaTrpF1, AvSerR1, MaSerR1) flanking the mitochondrial gene COI were used to amplify ~1900bp. This amplicon was used as the template in a PCR amplifying the 658bp ‘barcoding’ region of COI, using primers VFmix (1 VF1:1 VF1d:2 VF1i) and 5′ biotinylated VRmix (1 VR1:1 VR1d:2 VR1i). Labeled PCR products were mixed with the 15 host species-specific microsphere probes (Table 1). If probe-product hybridization was detected by the Luminex200®, a positive match was made. Unidentified PCR products were sequenced using the forward primer VF1d. Sequences were subsequently identified using the Barcode of Life Data Systems (BOLD; www.boldsystems.org).