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Ratio of sample median fluorescence intensity (MFI) to background MFI of 15 species-specific microsphere-attached probes tested against known vertebrate DNA sources and measured by the Luminex® 200. 200ng PCR product was added to each reaction, and fluorescence was measured for 100 microspheres of each probe to determine the median value. All highlighted cells have an MFI of >5X the background MFI. Black cells represent ‘true’ matches, and gray cells show non-specific hybridization.
