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. Author manuscript; available in PMC: 2012 Feb 8.
Published in final edited form as: Curr Neurovasc Res. 2012 Feb 1;9(1):20–31. doi: 10.2174/156720212799297137

Fig. 2. Wnt1 increases and maintains expression of WISP1 with neuronal cell injury blocked by WISP1 during OGD.

Fig. 2

(A) Hippocampal neuronal protein extracts (50 μg/lane) were immunoblotted with anti-WISP1 at 1, 3 and 24 hours following a 3 hour period of OGD. WISP1 expression was increased at 1, 3 and 24 hours following OGD and was further significantly increased by application of Wnt1 (100 ng/ml) 1 hour prior to OGD (*p<0.01 vs. control; †p <0.01 vs. OGD of corresponding time point). Quantification of western band intensity from 3 experiments was performed using the public domain NIH Image program (http://rsb.info.nih.gov/nih-image). (B) Increasing concentrations (1, 5, 10, and 20 ng/ml) of WISP1 protein were applied to neuronal cultures 1 hour prior to a 3 hour period of OGD and cell injury was determined by trypan blue (TB) dye exclusion and TUNEL 24 hours following OGD. WISP1 protected neurons against OGD in a concentration dependent manner, reducing trypan blue staining and DNA fragmentation at the concentrations of 10 ng/ml and 20 ng/ml (*p<0.01 vs. control; †p < 0.01 vs. OGD). Each data point represents the mean and SEM from 6 experiments.