Table 1.
Metabolites | MW (amu) | Time segment | SRM transition Q1 → Q3 (m/z) / | Collision energy (V) | Rt (min) |
---|---|---|---|---|---|
Quinolinic acid | 431.2 | 1 | 431.0 → 298.0 | 10 | 8.8 |
3-Hydroxykynurenine | 794.3 | 2 | 630.0 → 219.0 | 10 | 12.3 |
L-Kynurenine | 632.3 | 3 | 612.0 → 442.0 | 10 | 12.5 |
L-Tryptophan | 628.3 | 3 | 608.2 → 351.0 | 20 | 12.5 |
[2H3]Quinolinic acid | 434.2 | 1 | 434.0 → 301.0 | 10 | 8.8 |
[2H6]L-Kynurenine | 638.3 | 3 | 618.0 → 447.0 | 10 | 12.5 |
[2H5]L-Tryptophan | 633.3 | 3 | 613.2 → 351.0 | 20 | 12.5 |
MW: Molecular weight of analytes and deuterated standards after derivatization; Rt: Retention time; Q1, Q3: Quadrupoles. “Time segment” refers to the acquisition time periods depicted in Fig. 3.