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C3/α-2M thioester protein labelling in blood, plasma, serum and PEG-plasma. Blood, plasma, serum and PEG-plasma incubated with (+) or without (−) biotin–PEG4–hydrazide for 1 h at 52 °C. (A) Biotinylated proteins were detected by SA-HRP Western blot. Non-reducing (left panel) and reducing (right panel) conditions. (B) SDS-PAGE showing total protein content detected by Coomassie staining.
