Fig. 2.

Short-term exposure to extracellular or intracellular 50 μM curcumin does not affect ICl_swell. ICl_swell was activated in human renal cells (HEK293 Phoenix) by hypotonic shock and measured by the patch clamp technique in whole cell configuration. (a) Current density-to-voltage relations measured in extracellular hypertonic solution (hyper), at the maximal activation of the current in extracellular hypotonic solution (or steady state (hypo ss)), and after a 10 min exposure to an extracellular hypotonic solution containing 50 μM curcumin. (b) Current density-to-voltage relations measured in extracellular hypertonic solution (hyper), at the maximal activation of the current in extracellular hypotonic solution (or steady state (hypo ss)) and after a 10 min exposure to an extracellular hypotonic solution containing 0.5% DMSO as the vehicle. The current is not affected by a 10 min exposure to curcumin or DMSO (paired Student's t-test). (c) Current density-to-time graph showing the current in extracellular hypotonic solution containing 50 μM curcumin or 0.5% DMSO; the current is not affected by a 10 min exposure to curcumin or DMSO (paired Student's t-test). (d) Current density-to-voltage relation measured in extracellular hypertonic solution (hyper) and after a 10 min exposure to extracellular hypotonic solution (10 min hypo) in cells where 50 μM curcumin or 0.5% DMSO was added to the pipette filling (intracellular) solution. Data were fitted with second order polynomials (dotted line), following application of the extra-sum of squares F test and showed no effect of intracellular, short-term incubation with 50 μM curcumin.