Fig. 9.

Long-term exposure to curcumin increases the side scatter and volume of HT-29 cells in a dose-dependent manner. The effect of long-term exposure (22 h in a complete medium) to 5.0–50 μM curcumin on the side scatter and cell volume was investigated in HT-29 cells by flow cytometry. Control cells were incubated with 0.05% DMSO. Bar diagrams of (a) the mean side scatter of the whole population of cells and the mean cell volume (MCV, fl) of (b), necrotic and (c) late apoptotic cells. *p < 0.05, **p < 0.01, one way ANOVA with Dunnet's post-test or unpaired Student's t-test. Data were collected from at least four independent experiments.