Fig. 10.

Long-term exposure to curcumin induces G1-phase arrest and S-phase depression in HT-29 cells. The cell cycle distribution of HT-29 cells after long-term (22 hours) exposure to 5.0–20 μM curcumin added to the cell culture medium was assessed by analyzing isolated nuclei stained with DAPI using flow cytometry. Control cells were incubated with 0.05% DMSO. (a) Histograms showing the distribution of single nuclei in the G1, S and G2 phase in absence and presence of 5.0, 10, or 20 μM curcumin. (b) Bar diagrams show the percentages of single nuclei in the G1, S and G2 phase. *p < 0.05, unpaired Student's t-test. Data were collected from at least four independent experiments.