Skip to main content
. 2012 Feb 7;2:250. doi: 10.1038/srep00250

Figure 2. GHR-IR cells represent a distinct population of cells that exhibit extensive proliferation and self-renewal in vitro.

Figure 2

Contour plot of cells harvested from the PVR of adult mice incubated with secondary alone (PE conjugated goat-anti-rabbit IgG) (A) or anti-mouse GHBP (B) reveals a distinct population of viable GHBP+ve cells comprising 0.29±0.03% (mean±SEM, n = 9) of the total population. High power magnification of a typical GHBP-IR cell illustrates the punctate nature of labeling (Inset, B). (C) Relative expression levels of GHR transcript to GADPH in GHR+ve and GHR−ve sort populations harvested from adult SVZ (n = 3 independent experiments, mean ± SEM). (D) Individual clonally derived neurospheres from sorted GHR+ve cells are multipotent, generating neurons (red, β-III-tubulin), astrocytes (blue, GFAP), and oligodendrocytes (green, O4). Scale bar = 10 μm.