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. Author manuscript; available in PMC: 2013 Feb 20.
Published in final edited form as: Virology. 2011 Dec 29;423(2):175–186. doi: 10.1016/j.virol.2011.12.001

Figure 1. DC-SIGN receptor expression on Raji/DC-SIGN cells.

Figure 1

(A) Raji/DC-SIGN and Raji cells were stained with phycoerythrin (PE)-labeled mouse anti-human CD209 (DC-SIGN) and analyzed by flow cytometry. DC-SIGN expression is shown beneath the bar labeled M1. (B) HIV-1 subtype B, QH0692.42 and subtype C, COT6.15 were captured by Raji/DC-SIGN and Raji cells at 3 different cell concentrations. The amount of captured virus was measured by p24 ELISA. (C) Inhibition of HIV-1 binding to Raji/DC-SIGN cells by mouse anti-human CD209 antibody. The amount of captured virus was measured by p24 ELISA.