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. Author manuscript; available in PMC: 2013 Feb 20.
Published in final edited form as: Virology. 2011 Dec 29;423(2):175–186. doi: 10.1016/j.virol.2011.12.001

Figure 4. Inhibition of DC-SIGN mediated HIV-1 transfer by post and pre-DC-SIGN binding methods.

Figure 4

(A) In the post-DC-SIGN binding method, HIV-1 subtype B QH0692.42 and subtype C COT9.6 were incubated with Raji/DC-SIGN cells prior to the addition of GRFT, CV-N and SVN and co-culture with TZM-bl cells. (B) In the pre-DC-SIGN binding method, the virus was first incubated with GRFT, CV-N and SVN prior to the addition of Raji/DC-SIGN cells and co-culture with TZM-bl cells. The inhibition of transfer for both methods was determined by measuring the RLU. Bars represent mean ± SD of three different experiments. Untreated controls are shown in black.