Table 2.
Inhibition of HIV-1 transfer to TZM-bl cells via the DC-SIGN receptor
| HIV-1 envelope Pseudovirus |
a Post-DC-SIGN binding method | b Pre-DC-SIGN binding method | ||||
|---|---|---|---|---|---|---|
| GRFT | CV-N | SVN | GRFT | CV-N | SVN | |
| Subtype C | ||||||
| COT9.6 | 4.8 ± 0.5 | 31.5 ± 7.3 | 70.6 ± 14.6 | 19.4 ± 4.2 | 154.1 ± 25.6 | 230.5 ± 9.8 |
| CAP63.A9J | 35.0 ± 5.4 | 24.0 ± 5.6 | 441.3 ± 62.9 | 38.7 ± 5.2 | 33.7 ± 3.7 | 590.0 ± 82.4 |
| Du156.12 | 6.4 ± 0.4 | 69.2 ± 8.3 | 161.9 ± 11.6 | 28.8 ± 8.3 | 136.8 ± 73.3 | 284.0 ± 65.8 |
| Du151.2 | 4.8 ± 0.3 | 30.8 ± 3.2 | 82.8 ± 8.7 | 11.4 ± 1.0 | 131.5 ± 46.0 | 386.5 ± 71.3 |
| COT6.15 | 5.0 ± 0.1 | 31.2 ± 0.6 | 75.9 ± 0.8 | 18.6 ± 4.5 | 151.3 ± 22.7 | 149.7 ± 36.3 |
| Median | 5.0 | 31.2 | 82.8 | 19.4 | 136.8 | 284.0 |
| Subtype B | ||||||
| CAAN5342.A2 | 5.1 ± 0.1 | 18.8 ± 1.7 | 115.0 ± 2.4 | 7.1 ± 0.5 | 24.2 ± 2.1 | 153.0 ± 9.7 |
| PVO.4 | 4.4 ± 0.2 | 35.7 ± 2.1 | 82.4 ± 3.0 | 17.6 ± 2.7 | 162.8 ± 1.1 | 308.6 ± 16.2 |
| QH0692.42 | 5.3 ± 0.1 | 36.9 ± 1.4 | 77.3 ± 5.7 | 21.7 ± 8.3 | 135.4 ± 18.3 | 438.4 ± 58.8 |
| Median | 5.1 | 35.7 | 82.4 | 17.6 | 135.4 | 308.6 |
a,b
Results are shown as IC50 (nM) which is the concentration needed to inhibit HIV-1 transfer by 50%.