Table 2.
Area fractions of DPPC/DOPC/ergosterol samples in PBS-20 vol % ethanol buffer with 0.5 mol % TR-DHPE
| DPPC/DOPC | Erg (mol %) | Af Lα | Af Lo | Af LβI |
|---|---|---|---|---|
| 1:4 | 0 | 1.00 | — | — |
| 1:4 | 10 | 1.00 | — | — |
| 1:4 | 30 | 1.00 | — | — |
| 2:3 | 0 | 0.75 ± 0.08 | — | 0.25 ± 0.08 |
| 2:3 | 10 | 0.90 ± 0.02 | 0.05 ± 0.01 | 0.05 ± 0.02 |
| 2:3 | 20 | 0.95 ± 0.01 | 0.05 ± 0.01 | — |
| 2:3 | 30 | 0.93 ± 0.01 | 0.07 ± 0.01 | — |
| 3:2 | 0 | 0.42 ± 0.10 | — | 0.58 ± 0.10 |
| 3:2 | 10 | 0.53 ± 0.13 | 0.04 ± 0.03 | 0.43 ± 0.15 |
| 3:2 | 20 | 0.57 ± 0.12 | 0.12 ± 0.04 | 0.31 ± 0.16 |
| 3:2 | 30 | 0.73 ± 0.04 | 0.26 ± 0.05 | 0.01 ± 0.02 |
| 3:2 | 45 | 0.68 ± 0.02 | 0.32 ± 0.02 | — |
| 3:2 | 55 | 0.73 ± 0.02 | 0.27 ± 0.02 | — |
| 4:1 | 10 | 0.29 ± 0.08 | 0.03 ± 0.02 | 0.68 ± 0.09 |
| 4:1 | 20 | 0.39 ± 0.04 | 0.15 ± 0.02 | 0.46 ± 0.05 |
| 4:1 | 30 | 0.38 ± 0.06 | 0.27 ± 0.03 | 0.35 ± 0.08 |
| 9:1 | 0 | 0.25 ± 0.15 | — | 0.75 ± 0.15 |
| 9:1 | 10 | 0.16 ± 0.03 | 0.07 ± 0.01 | 0.77 ± 0.04 |
| 9:1 | 20 | 0.22 ± 0.03 | 0.16 ± 0.02 | 0.62 ± 0.05 |
| 9:1 | 30 | 0.19 ± 0.03 | 0.39 ± 0.04 | 0.42 ± 0.04 |
| 9:1 | 45 | 0.23 ± 0.04 | 0.77 ± 0.04 | — |
| 1:0 | 10 | — | 0.10 ± 0.01 | 0.90 ± 0.01 |
| 1:0 | 20 | — | 0.23 ± 0.03 | 0.77 ± 0.03 |
| 1:0 | 30 | — | 0.42 ± 0.02 | 0.58 ± 0.02 |
Area fractions averaged from 10–15 images obtained from two to four independent samples. Error estimates are the standard deviation from the mean.