Figure 1.

(A–D) CHO-K1 cell stably expressing monomeric EGFP, with the chromatin marked by H2B-mCherry in four nuclear stages identified as being structurally distinct from mid-mitosis to early interphase. Scale bar: 3 μm. (E–H) Overlay of free EGFP and H2B-mCherry localization in the plane of the 3.2 μm line drawn in the four stages identified in A–D, respectively. (I–L) Overlay of the intensity profile of the H2B-mCherry stain with the fluorescence intensity carpet of the line drawn across freely diffusing EGFP (10⁵ lines) in E–H, respectively. (M–P) The pCF carpet derived from the intensity carpets constructed in I–L at a distance measuring EGFP molecular flow into and out of the most compact chromatin regions present along the respective line.