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. 1981 Oct 10;9(19):4981–4995. doi: 10.1093/nar/9.19.4981

The endogenous proviral mouse mammary tumor virus genes of the GR mouse are not identical and only one corresponds to the exogenous virus.

P Herrlich, N E Hynes, H Ponta, U Rahmsdorf, N Kennedy, B Groner
PMCID: PMC327493  PMID: 6273791

Abstract

The endogenous proviral copies of mouse mammary tumor virus (MMTV) were selected from a gene library of GR mouse DNA. We obtained five different lambda. MMTV recombinant clones. Four of them correspond to the 3' Eco RI fragments of the endogenous proviruses an one comprises an intact MMTV provirus with 2 to 3 kb of flanking mouse genomic DNA. Heteroduplex formation followed by S1 digestion under stringent conditions shows that there is nucleotide sequence heterology among the cloned endogenous proviral copies. Only one endogenous proviral copy, associated with the mtv-2 locus, was found to be totally homologous to the exogenous proviral DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arthur L. O., Altrock B. W., Schochetman G. Type-specific determinants on proteins of an endogenous C3H mouse mammary tumor virus (MMTV) distinguish this virus from highly oncogenic exogenous MMTVs. Virology. 1981 Apr 30;110(2):270–280. doi: 10.1016/0042-6822(81)90059-3. [DOI] [PubMed] [Google Scholar]
  2. Bentvelzen P., Brinkhof J., Haaijman J. J. Genetic control of endogenous murine mammary tumour viruses reinvestigated. Eur J Cancer. 1978 Oct;14(10):1137–1147. doi: 10.1016/0014-2964(78)90070-1. [DOI] [PubMed] [Google Scholar]
  3. Cohen J. C., Majors J. E., Varmus H. E. Organization of mouse mammary tumor virus-specific DNA endogenous to BALB/c mice. J Virol. 1979 Nov;32(2):483–496. doi: 10.1128/jvi.32.2.483-496.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cohen J. C., Shank P. R., Morris V. L., Cardiff R., Varmus H. E. Integration of the DNA of mouse mammary tumor virus in virus-infected normal and neoplastic tissue of the mouse. Cell. 1979 Feb;16(2):333–345. doi: 10.1016/0092-8674(79)90010-2. [DOI] [PubMed] [Google Scholar]
  5. Cohen J. C., Varmus H. E. Endogenous mammary tumour virus DNA varies among wild mice and segregates during inbreeding. Nature. 1979 Mar 29;278(5703):418–423. doi: 10.1038/278418a0. [DOI] [PubMed] [Google Scholar]
  6. Fanning T. G., Puma J. P., Cardiff R. D. Identification and partial characterization of an endogenous form of mouse mammary tumor virus that is transcribed into the virion-associated RNA genome. Nucleic Acids Res. 1980 Dec 11;8(23):5715–5723. doi: 10.1093/nar/8.23.5715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fanning T. G., Puma J. P., Cardiff R. D. Selective amplification of mouse mammary tumor virus in mammary tumors of GR mice. J Virol. 1980 Oct;36(1):109–114. doi: 10.1128/jvi.36.1.109-114.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Friedrich R., Morris V. L., Goodman H. M., Bishop J. M., Varmus H. E. Differences between genomes of two strains of mouse mammary tumor virus as shown by partial RNA sequence analysis. Virology. 1976 Jul 15;72(2):330–340. doi: 10.1016/0042-6822(76)90162-8. [DOI] [PubMed] [Google Scholar]
  9. Groner B., Buetti E., Diggelmann H., Hynes N. E. Characterization of endogenous and exogenous mouse mammary tumor virus proviral DNA with site-specific molecular clones. J Virol. 1980 Dec;36(3):734–745. doi: 10.1128/jvi.36.3.734-745.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Groner B., Hynes N. E. Number and location of mouse mammary tumor virus proviral DNA in mouse DNA of normal tissue and of mammary tumors. J Virol. 1980 Mar;33(3):1013–1025. doi: 10.1128/jvi.33.3.1013-1025.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hohn B., Murray K. Packaging recombinant DNA molecules into bacteriophage particles in vitro. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3259–3263. doi: 10.1073/pnas.74.8.3259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hynes N. E., Kennedy N., Rahmsdorf U., Groner B. Hormone-responsive expression of an endogenous proviral gene of mouse mammary tumor virus after molecular cloning and gene transfer into cultured cells. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2038–2042. doi: 10.1073/pnas.78.4.2038. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Massey R. J., Arthur L. O., Nowinski R. C., Schochetman G. Monoclonal antibodies identify individual determinants on mouse mammary tumor virus glycoprotein gp52 with group, class, or type specificity. J Virol. 1980 Jun;34(3):635–643. doi: 10.1128/jvi.34.3.635-643.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Michalides R., van Nie R., Nusse R., Hynes N. E., Groner B. Mammary tumor induction loci in GR and DBAf mice contain one provirus of the mouse mammary tumor virus. Cell. 1981 Jan;23(1):165–173. doi: 10.1016/0092-8674(81)90281-6. [DOI] [PubMed] [Google Scholar]
  15. Mühlbock O. Note on a new inbred mouse-strain GR-A. Eur J Cancer. 1965 Oct;1(2):123–124. doi: 10.1016/0014-2964(65)90003-4. [DOI] [PubMed] [Google Scholar]
  16. Ringold G., Lasfargues E. Y., Bishop J. M., Varmus H. E. Production of mouse mammary tumor virus by cultured cells in the absence and presence of hormones: assay by molecular hybridization. Virology. 1975 May;65(1):135–147. doi: 10.1016/0042-6822(75)90014-8. [DOI] [PubMed] [Google Scholar]
  17. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]

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