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Effects of RTV and NDGA on eNOS mRNA levels in porcine pulmonary arteries and HPAECs. The vessel rings or HPAECs were cultured with DMEM and DMSO (control) or treated with RTV (15 μmol/L) alone or together with NDGA (1.75 or 3.50 μmol/L) for 24 hours. eNOS mRNA levels were determined by real-time PCR and normalized to CD31 or GAPDH mRNA. (A) eNOS mRNA levels in porcine artery rings. (B) eNOS mRNA levels in HPAECs. Values are means ±SEM, n=3, * P<0.05.
