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. 2011 Nov 1;17(11):BR312–BR318. doi: 10.12659/MSM.882040

Figure 4.

Figure 4

Effects of RTV and NDGA on ROS production in porcine pulmonary arteries and HPAECs. The vessel rings or HPAECs were treated with DMEM and DMSO (control), RTV (15 μmol/L), RTV (15 μmol/L) together with NDGA (3.50 μmol/L), or NDGA (3.5 μmol/L) for 24 hours. (A) Superoxide anion levels at the endothelial layer were determined with the lucigenin-enhanced chemiluminescence assay. (B) GSH levels in HPAECs were assayed using a GSH-mediated quantification kit. GHS levels reversely correlate to cellular ROS levels. Values are means ±SEM, n=4, * P<0.05.