FIG. 5.

MDA-7- induced apoptosis in tumor cells but not normal cells. Tumor cells (MCF-7, HeLa, HepG2) and NHLF cells were transfected with pCDNA3.1, pCDNA3.1/IL-24, pCDNA3.1/RGD-IL-24, or treated with the medium (control). Cells were analyzed for apoptosis by Annexin V assay and Hoechst 33258. (A) Prophase apoptotic cells were recognized by binding with FITC on the membrane. (B) Seventy-two hours after transfection, apoptotic cells were recognized by condensation of nuclear chromation and its fragmentation. *P<0.05 versus pCDNA3.1/IL-24. Color images available online at www.liebertonline.com/jir