Fig. 1.

A: representative Western blot using antibodies against murine (endogenous) ectonucleotide pyrophosphate phosphodiesterase (ENPP1) protein at 110 kDa in epididymal adipose tissue of adipose ENPP1 transgenic (AdiposeENPP1-TG) mice and wild-type littermates after 60% fat diet with pair-feeding protocol. The optical density is expressed in arbitrary units (AU) as means ± SD for each study group. B: representative Western blot for total and phospho-insulin receptor Tyr¹³⁶¹ (95 kDa) in adipose tissue. C: representative Western blot for total and phospho-Akt Ser⁴⁷³ (60 kDa) in adipose tissue. Tissue was collected after food was withheld for 5 h. Fasting plasma insulin concentrations, at the time of adipose tissue collection, are also shown.