Fig. 5.

A: representative Western blot using antibodies against murine (endogenous) ENPP1 protein at 110 kDa in liver of AdiposeENPP1-TG mice and wild-type littermates exposed to 12 wk of a 60% fat diet with pair-feeding protocol. The optical density is expressed in AU as means ± SD for each study group. B: representative Western blot for total and phospho-insulin receptor Tyr¹³⁶¹ (95 kDa) of liver. C: representative Western blot for total and phospho-Akt Ser⁴⁷³ (60 kDa). Tissue was collected after food was withheld for 5 h. Fasting plasma insulin concentrations, at the time of adipose tissue collection, are also shown in B and C.