Table 1.
List of primers used in qPCR for chondrogenic genes (www.ncbi.nlm.nih.gov/nucleotide).
Chondrogenic Gene | Gene Bank Accession No. | Primer sequences |
Human Collagen Type I, Col I | NM_000088 | F: 5′-agg gct cca acg aga tcg aga-3′R: 5′-tac agg aag cag aca ggg cca-3′ |
Human Collagen Type II, Col II | NM_001844 | F: 5′-cta tct gga cga agc agc tgg ca-3′R: 5′-atg ggt gca atg tca atg atg g-3′ |
Human Collagen Type X, Col X | NM_000493 | F: 5′-gct aag ggt gaa agg ggt tc-3′R: 5′-ctc cag gat cac ctt ttg ga-3′ |
Human Collagen Type XI, Col XI | NM_080681 | F: 5′-agc agg ggg tga gac ctg tg -3′R: 5′-tgc tcc aga gca ggg gta gg-3′ |
Human Aggrecan Core Protein, ACP | NM_001135 | F: 5′-cac tgt tac cgc cac ttc cc-3′R: 5′-acc agc gga agt ccc ctt cg-3′ |
Human Sox9, SOX9 | NM_000346 | F: 5′-gcg gag gaa gtc ggt gaa ga-3′R: 5′-ccc tct cgc ttc agg tca gc-3′ |
Cartilage Oligomeric Protein, COMP | NM_000095 | F: 5′-aac tca ggg cag gag gat gt-3′R: 5′-tgt cct ttt ggt cgt cgt tc-3 |
ELASTIN | NM_000501 | F: 5′-ggc ctg gag gca aac ctc tt-3′R: 5′-cca cca act cct ggg aca cc-3′ |
GAPDH | NM_002046 | F: 5′-tcc ctg agc tga acg gga ag-3′R: 5′-gga gga gtg ggt gtc gct gt-3′ |