Fig. 2.

Ins regulates menin expression acutely via the Akt pathway followed by MAPK. A: protein expression of menin, p-Akt, Akt, and GAPDH in HepG2 lysates after overnight serum starvation was determined under the conditions of either 1-h pretreatment of LY-294002 (10 μM), Ins treatment (100 nM), or a combination of both as indicated by the + and − signs at the allotted time points (LY-294002 treatment alone was collected 1 h post-LY-294002 administration). B: quantification of Western blots from A by densitometry showing menin relative to GAPDH. C: protein expression of menin, p-ERK, ERK, and GAPDH in HepG2 lysates after overnight serum starvation was determined under the conditions of either 1-h pretreatment of U-O126 (10 μM), Ins treatment (100 nM), or a combination of both as indicated by + or − signs at the allotted time points (U-O126 treatment alone was collected 1 h post-U-O126administration). D: quantification of Western blots from C by densitometry showing menin relative to GAPDH.