Table 1.
Primer sets used in RT-PCR
| Gene | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
| β-Actin | ACGGCTCCGGCATGTGCAAG | TGACGATGCCGTGCTGCATG |
| ASAH1 | GCACAAGTTATGAAGGAAGCCAAG | TCCAATGATTCCTTTCTGTCTCG |
| ASAH2 | GCATCAACACAGGAGAGTC | GGAGGCAGAGGCATAGAG |
| ACER3 | ATCCGCCTGGTCTTCATC | CTCCTTATTGCTGGTCTTCC |
| SK1 | CTGGCAGCTTCCTTGAACCAT | TGTGCAGAGACAGCAGGTTCA |
| SK2 | CCAGTGTTGGAGAGCTGAAGGT | GTCCATTCATCTGCTGGTCCTC |
| SPTLC1 | AGTCCCTTTCTCCAGCCTTTC | TTCCACCGTGACCACAACC |
| SPTLC2 | CAAGAAGAAATACAAGGCATAC | CCATCATAACATCCACATCC |
| LASS1 | CCTTCTACTTCTTCTTCAATGC | TCGGCTGTGTCATACTCC |
| LASS2 | GTCATCCTGCCCTTCTGG | CACTGCTGGCATCTTCTACC |
| LASS3 | TCATACATCTTCCTCAACCTACAG | GCCTCTTCTTCTTCCTCTTCC |
| LASS5 | ATGGTGGCTCCTCAATGG | AGGTGGTCACATCTTCTTCC |
| LASS6 | GCTGACGAGGTTCTGTGAG | AGTTGTGAGTGGCTGATAGG |
| ELOVL1 | GTCTACAACTTCTCACTGGTGGC | AAGTGCCTCAGGGCTGTTGGAA |
| ELOVL2 | TCCACTTGGGAAGGAGGCTACA | CCAGGAACTCTACTGATTTGGAG |
| ELOVL4 | CCGAGAACCTTTTCAGATGCGTC | AATCCACACTCTGGCAAATATAG |
| ELOVL5 | ACGTCTACCACCATGCCTCGAT | TGGAAGGGACTGACGACAAACC |
| ELOVL6 | CCATCCAATGGATGCAGGAAAAC | CCAGAGCACTAATGGCTTCCTC |
| ELOVL7 | CCTACTATGGACTTTCTGCATTGG | GAACTGGCTTATGTGGATGGCG |
| CYP17A1 | CTCTTGCTGCTTCACCTA | TCAAGGAGATGACATTGGTT |
| CYP11A1 | CGTGGAGTCGGTTTATGTC | CTCTGGTAATACTGGTGATAGG |
| CYP11B1/2a | ACGGCGACAACTGTATCC | AGAGCGTCATCAGCAAGG |
| CYP21A2 | TGTGGAACTGGTGGAAGC | GGTGGAGCCTGTAGATGG |
| 3β-HSD | CCAGTAGCATAGAGGTAGCC | TCAGATTCCACCCGTTAGC |
| NR5A1 | GGAGTTTGTCTGCCTCAAGTTCA | CGTCTTTCACCAGGATGTGGTT |
| NR5A2 | TACCGACAAGTGGTACATGGAA | CGGCTTGTGATGCTATTATGGA |
| NR4A1 | GGACAACGCTTCATGCCAGCAT | CCTTGTTAGCCAGGCAGATGTAC |
| NR4A2 | AAACTGCCCAGTGGACAAGCGT | GCTCTTCGGTTTCGAGGGCAAA |
| NR4A3 | ACTGCCCAGTAGACAAGAGACG | GTTTGGAAGGCAGACGACCTCT |
| NR0B1 | CCAAATGCTGGAGTCTGAACATC | CCCACTGGAGTCCCTGAATGTA |
| NR0B2 | TGCCTGAAAGGGACCATCCTCT | GTTCCAGGACTTCACACAGCAC |
| StAR | GCTCTCTACTCGGTTCTC | GCTGACTCTCCTTCTTCC |
| TSPO | GCAGATTCCGTGATTACAGTG | TCCTCCTCGTCGTCATCG |
| HSL | CACTACAAACGCAACGAGAC | CCAGAGACGATAGCACTTCC |
| MC2R | GTGGTGCTTACGGTCATCTGGA | AGGCACAGGATGAAGACCAGCA |
| PDE4B | TAGTCAGCCTCCTGTCTCCAGA | GAAGCCATCTCACTGACAGACC |
| SGPP2 | ACTCCTCATCGTCCTCAC | CAGAATCCTATGGTCACTCC |
| nSMase 1 | GTGCTCAACGCCTATGTG | GTCTGCCTTCTTGGATGTG |
| nSMase 2 | ACTGCTCCTCTGACGACAAG | CCACGGCTTCTCCTCACC |
| SGMS2 | ACTCTACCTGTGCCTGGAATG | AATAAGTCAGTGTCAGCGTAACC |
| aSMase 1 | TTGTAGCCAGGTATGAGAAC | GCCGATGTAGGTAGTTGC |
| CERK | ACCACTGACATCATCGTTACTG | GCACCTCGCTGAACATACC |
| DEGS1 | GTTTGGAATGTTTGCTAATC | GAGAGGCTGAAGAATAACC |
a
The primer set used to assess the mRNA expression of CYP11B1/2 cannot distinguish between CYP11B1 and CYP11B2 isoforms.