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FasL activation of CASP3 is accompanied by elevated levels of the antiapoptotic protein MCL1. Cytoplasmic extracts from hTERT cells were analyzed for levels of active CASP3 and MCL1 by immunoblotting after exposure to FasL (0, 1, 5, 10, 50, and 100 ng/ml) for 24 h after UV exposure (0, 10, 100, and 500 mJ/cm2). PDI was used as our loading control. These data are representative of three independent experiments (n = 3).
