Fig. 10.

Ablation of myometrial MCL1 levels induced FasL-mediated CASP3 and PARP cleavage. hTERT cells were transfected with MCL1 siRNA for 24 h and treated for an additional 12 h with FasL. Cytoplasmic extracts were analyzed for MCL1 protein levels by immunoblot for the following experimental conditions: scrambled siRNA control, MCL1 siRNA, FasL plus scrambled siRNA, and FasL plus MCL1 siRNA. Cytoplasmic extracts from the same conditions were analyzed for active CASP3. Nuclear extracts from the same conditions were analyzed for cleaved PARP. PDI and NCOA3 were used as loading controls. These data are representative of three independent experiments (n = 3).