Fig. 11.

Ablation of myometrial MCL1 levels induced FasL-mediated TUNEL-positive staining in CASP3-positive hTERT cells. hTERT cells were transfected with MCL1 siRNA and treated with FasL for 12 h. Scrambled siRNA control, FasL plus scrambled siRNA, MCL1 siRNA, and FasL plus MCL1 siRNA were analyzed by fluorescent microscopy after TUNEL staining (green) to detect the cleavage of nuclear DNA. Cells were also stained for cleaved CASP3 (red), and nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). These data are representative of three independent experiments (n = 3).