Fig. 6.

Increased TUNEL staining was detected in UV- but not FasL-treated CASP3-positive hTERT cells. hTERT myometrial cells were examined 24 h after UV and FasL exposure and analyzed by fluorescent microscopy after TUNEL staining (green) to detect the presence of fragmented nuclear DNA. Cells were also stained for cleaved CASP3 (red), and nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Vehicle-treated hTERT cells were used as a negative control. These data are representative of three independent experiments (n = 3).