Fig. 6.

Fat metabolism in primary hepatocytes from CaMKK2 KO mice is enhanced. Hepatocytes from CaMKK2 KO mice show increased rates of de novo lipogenesis (A) and fatty acid oxidation (B). To determine glucokinase mRNA levels and lactate production, hepatocytes were cultured overnight in hepatocyte media with 25 mm glucose. The next morning control cells or those treated with NA for 5 h were harvested, and the quantity of glucokinase mRNA, normalized for cyclophilin, was determined by RT-PCR (C). Cell culture media were collected overnight, and lactate was quantified using L-Lactate Assay Kit (Eton Bioscience, Inc.) (panel D). [n = 3 (panel A), n = 5 (panel B), n = 3 (panel C), and n = 3 (panel D)].