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. 2012 Feb 6;196(3):327–335. doi: 10.1083/jcb.201107115

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© 2012 Christis and Munro

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Figure 2. — Sec71 binds to Arl1-coated liposomes in a GTP-dependent manner. (A) Comparison of the peptide spectral counts for proteins bound to liposomes coated with GTP- or GDP-locked forms of ARFRP1. (B) As in A, except for Arl1. Known effectors dGolgin-245 and dGCC88 are shown (closed red circles) along with Sec71 (open red circles), and proteins among the top 100 found on liposomes are indicated in purple. (A and B) The graphs are representative of two independent experiments. (C) Anti-GFP immunoblot of a small-scale liposome-binding assay using lysates (Ly) of S2 cells expressing Sec71-GFP as input and the indicated forms of Arl1 or Arf1. Li, liposomes; Su, subnatant. Molecular mass is indicated in kilodaltons.