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. 2011 Dec 20;153(2):700–711. doi: 10.1210/en.2011-0247

Fig. 7.

Fig. 7.

GnRHa- or CRH-induced transcriptional up-regulation of Nur77 in primary mixed pituitary cell cultures from mice with pituitary-targeted ablation of either c-Raf or ERK1/2. A, Whole pituitaries from mice with αGSU-CRE-mediated pituitary-specific disruption of c-Raf (c-Raf conditional knockout, c-Raf CKO) were dispersed into primary culture. After serum starvation (2 h), cultures were exposed to either vehicle and GnRHa or CRH for 60 min. Nur77 mRNA levels were measured by qPCR. Bars represent mean ± sem for three replicates within an individual experiment. This experiment was conducted on three separate occasions. Bars with different letter designations represent mean values that are statistically significantly different at P < 0.05. B, Whole pituitaries from mice with pituitary-specific compound ablation of ERK1 and ERK2 (ERK1/2 double knockout, ERK1/2 DKO) were dispersed into primary culture and treated as in A. Bars represent mean ± sem for three replicates within an individual experiment. This experiment was conducted on two separate occasions. Bars with different letter designations represent mean values that are statistically significantly different at P < 0.05.