Fig. 2.

VLDL effects on steroidogenic enzyme expression in H295R cells. A and B, Cells were treated with AngII (10 nm) or VLDL (100 μg/ml) for 24 h (A and B) or for the indicated time (C). RNA was isolated and reverse transcribed for qPCR quantification. qPCR data were normalized to 18S rRNA and shown as fold change compared with basal. C, Cells were lysed, and Western analysis was performed for StAR protein quantification. Western data were normalized to β-actin protein levels. Results represent the means ± sem of data from at least three independent experiments. Statistical analyses were performed using paired ANOVA followed by Dunnett's post hoc test. *, P < 0.05; **, P < 0.01, compared with the basal condition.