A and B, Results of the analysis of serum-free T4 (A) and T3 (B) levels in WT and PRCPgt/gt fed on a regular chow diet or overnight fasted. C and D, Results of TSH mRNA levels in the pituitary of fed and fasted WT and PRCPgt/gt mice and UCP1 mRNA expression levels in BAT of fed WT and PRCPgt/gt mice by real-time PCR. Data are expressed as means ± se; *, P < 0.05; **, P < 0.01; ***, P < 0.001.