Fig. 6.
A–D, Representative photographs of PVN from hypothyroid WT and PRCPgt/gt mice treated either with saline (A and B, respectively) or T3 (C and D, respectively) showing the silver grains from radioactive in situ hybridization of TRH mRNA on Nissl-stained background. 3v, Third ventricle. E, Results from the analysis of the expression levels of TRH mRNA from each group. TRH mRNA expression levels were measured using ImageJ program, and relative density of silver grains was compared between groups. **, P < 0.01. Scale bar in D, 50 μm (for A–D).