Fig. 5.

TR binding to target genes increases during natural metamorphosis and is accompanied by increased recruitment of RNA Pol II and reduced core histones at the TRE. The intestine was isolated from tadpoles at premetamorphosis (stage 54), early metamorphic climax (stage 58), metamorphic climax (stage 60–62), and the end of metamorphosis (stage 66) and subjected to ChIP assay with the anti-TR (A), anti-Pol II (B), anti-H3 (C), anti-H2B (D), or anti-Id14 (extracellular protein, as a negative control) (E) antibody. The ChIP DNA was analyzed as in Fig. 2. Note that TR binding to the TRE was higher during metamorphosis (stages 58–62), accompanied by increased Pol II and reduced amounts of core histones at TRE of both TR-target promoters at stages 58–62. Only background signals were observed with the anti-ID14 antibody. Error bars indicate sem.