Fig. 6.

Histone modifications associated with gene activation are increased at TR-target genes during natural metamorphosis. The ChIP assay was performed as in Fig. 5 on intestines isolated from tadpoles at the indicated stages with anti-H3K4me3 (A), anti-H3K79me3 (B), anti-H3R17me2a (C), anti-AcH3 (D), or anti-AcH4 antibody (E). ChIP signals were normalized with the ChIP signals of histone H3 in Fig. 5 for the corresponding promoter/exon regions. Note that in general, these activation histone marks were increased at the two promoters during metamorphosis. Error bars indicate sem.